Genomic Analysis of Homotypic Vacuole Fusion
نویسندگان
چکیده
منابع مشابه
Genomic analysis of homotypic vacuole fusion.
Yeast vacuoles undergo fission and homotypic fusion, yielding one to three vacuoles per cell at steady state. Defects in vacuole fusion result in vacuole fragmentation. We have screened 4828 yeast strains, each with a deletion of a nonessential gene, for vacuole morphology defects. Fragmented vacuoles were found in strains deleted for genes encoding known fusion catalysts as well as 19 enzymes ...
متن کاملIon regulation of homotypic vacuole fusion in Saccharomyces cerevisiae.
Biological membrane fusion employs divalent cations as protein cofactors or as signaling ligands. For example, Mg2+ is a cofactor for the N-ethylmaleimide-sensitive factor (NSF) ATPase, and the Ca2+ signal from neuronal membrane depolarization is required for synaptotagmin activation. Divalent cations also regulate liposome fusion, but the role of such ion interactions with lipid bilayers in Ra...
متن کاملPhosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion.
Yeast vacuoles undergo cycles of fragmentation and fusion as part of their transmission to the daughter cell and in response to changes of nutrients and the environment. Vacuole fusion can be reconstituted in a cell free system. We now show that the vacuoles synthesize phosphoinositides during in vitro fusion. Of these phosphoinositides, phosphatidylinositol 4-phosphate and phosphatidylinositol...
متن کاملThe SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.
The NSF homolog Sec18 initiates fusion of yeast vacuoles by disassembling cis-SNARE complexes during priming. Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified. Here we show that the SNARE Ykt6 mediates Vac8 palmitoylation and acts during a novel subreaction of vacuole fusion. This subreaction is controlled by a Sec17-i...
متن کاملYeast homotypic vacuole fusion requires the Ccz1–Mon1 complex during the tethering/docking stage
The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes could not fuse. Morphological evidence suggested that these mutant vacuoles could not proceed to ...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2002
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.01-10-0512